Advances in Applied Microbiology, Vol. 52 by Allen I. Laskin

By Allen I. Laskin

An eclectic quantity of topical reports on all points of utilized microbiology. It comprises 14 finished studies of present study in utilized microbiology.

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The bacterial DNA invertases are a particularly well-characterized family of closely related site-specific recombination systems (Dybvig, 1993), but each system encodes its own specialized recombinase that functions at RECOMBINASES IN WHOLE-CELL BIOSENSORS OF POLLUTION 43 unique DNA sites assisted by additional cellular proteins. All invertases are known to be able to complement each another (Plasterk and van de Putte, 1984a) and each system is discussed in detail below. 1. The Pin System A cryptic episome-like element has been found to be present in the E.

Industrial biocatalysis today and tomorrow. Nature 409, 258–268. , and Kobayashi, M. (1997). Screening of novel microbial enzymes for the production of biologically and chemically useful compounds. Adv. Biochem. Eng. Biotechnol. 58, 45–87. Short, J. M. (1997). Recombinant approaches for accessing biodiversity. Nat. Biotechnol. 15, 1322–1323. Short, J. M. (1999a). Enzyme kits and libraries. S. patent 6,004,788 issued to Diversa Corporation. Short, J. M. (1999b). Protein activity screening of clones having DNA from uncultivated microorganisms.

Laborious protocols to detect reporter gene output are time consuming and usually expensive; rapid inexpensive equipment for detection of reporter gene expression is therefore important. III. DNA Recombination as a Potentially Novel Reporter System Here we briefly review site-specific recombination and describe how it could be developed to produce a reporter for in situ gene expression. The advantages/disadvantages of recombinases for this purpose are discussed. The ability to respond rapidly to environmental changes is of paramount importance for microbial survival.

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