Advances in Applied Microbiology, Vol. 35 by Saul L. Neidleman (ed.), Allen I. Laskin (ed.)

By Saul L. Neidleman (ed.), Allen I. Laskin (ed.)

Meant for researchers in utilized microbiology and environmental engineers, this e-book covers such subject matters as environmental evaluate of biotechnological strategies and microbial changes of haloaromatic and haloaliphatic compounds.

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Ltd. 5 because of a reduction in substrate porosity, loss of the structure of WB, development of stickiness in the moist medium, and difficulty in oxygen transfer thereby making such a medium difficult to use, especially on a large scale. , 1960). , 1961; Park and Rivera, 1982; Tobey and Yousten, 1976). 17) was employed by Lulla and Subrahmanyan (1954). , R. K. LONSANE AND M. V. , 1961), or nutrient sporulation broth containing 8 g/liter DIFCO nutrient broth with 7 x M CaC12, 5 X M MnC12, and 1 x M MgClz (Tobey and Yousten, 1976).

I’ONSANE AND M. V. , 1982). 8. The moldy fermented solids are subjected to extraction immediately at the end of the fermentation or are air or vacuum dried. The dried bran can be stored for several months without any appreciable decrease in enzyme titer. It may be used as such, or the enzyme may be extracted whenever required. A survey of literature on the methodology adopted by various workers for extraction of bacterial a-amylase from bacterial bran produced in the SSF technique indicates that it is similar to the trends just described.

The chronological sequence of studies on bacterial a-amylase production under the SSF process is presented in Table I. B. , 1979; Underkofler, 1976). The bacterial cultures reported for the production of a-amylases in the SSF process are also limited to the genus Bacillus (Table 11). Beckord et al. (1945) studied 16 starch-hydrolyzing bacterial cultures for their ability to produce the enzyme in the SSF technique and reported that 11 cultures were able to produce a-amylases in varying amounts. Among these, the capability to produce high titers of the enzyme was confined to three species (B.

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